Evaluation of 1066 Toxcast Chemicals in a Human Stem Cell Assay for Developmental Toxicity [SOT 2015]
Presented at the Society of Toxicology’s 55th Annual Meeting and ToxExpo, March 2015, in San Diego, California.
EPA’s ToxCast program has generated data on a battery of 821 in vitro endpoints for 1066 compounds including pharmaceuticals, natural products, pesticidal active ingredients, consumer use chemicals and industrial ingredients. To increase the diversity of in vitro assays used to assess developmental toxicity, the ToxCast library was evaluated in the Stemina ‘devTOX quickPREDICT’ (qP) platform. This assay measures two small molecules (ornithine, cystine) in medium conditioned by human embryonic stem (hES) cells yielding an ornithine:cystine ratio (o/c ratio) indicative of an imbalance in metabolism predictive for teratogenicity in a human system.